Cytiva (GE Healthcare) BPG 200/500 AxiChrom Chromatography Column – Bioprocess / Protein Purification

Cytiva AxiChrom™ BPG 200/500 Chromatography Column

The Cytiva AxiChrom™ BPG 200/500 Chromatography Column is a high-performance preparative and process-scale chromatography column designed for demanding protein purification, biomolecule separation, and biopharmaceutical downstream processing applications. Originally developed under GE Healthcare Life Sciences and now part of the Cytiva portfolio, this column is widely trusted in biotech, pharmaceutical, and advanced research laboratories worldwide.

As part of the AxiChrom™ platform, the BPG 200/500 column is engineered to simplify column packing, improve reproducibility, and support seamless scale-up from pilot to production environments. Its axial compression design enables consistent packed bed quality, helping ensure reliable chromatographic performance across repeated runs and process transfers.

High-Efficiency Preparative Chromatography

The AxiChrom BPG 200/500 is optimized for preparative HPLC and process chromatography, supporting high-resolution separations for proteins, antibodies, enzymes, and other biomolecules. Its design promotes uniform flow distribution and stable bed packing, which are critical for achieving high yields and reproducible purification results in both development and manufacturing workflows.

Intelligent Packing & Automated Workflows

When integrated with Cytiva ÄKTA™ chromatography systems and UNICORN™ control software, the AxiChrom column supports automated axial packing and unpacking. This reduces manual handling, minimizes operator-to-operator variability, and shortens setup time, making it ideal for regulated environments where consistency and documentation are essential.

Robust, Process-Ready Construction

Built for long-term use in modern bioprocessing facilities, the BPG 200/500 column features durable, chemically resistant materials suitable for routine cleaning, sanitization, and repeated cycling. Its construction supports typical chromatography operating pressures and is compatible with commonly used resins and buffers in downstream processing.

Scalable Platform for Process Development & Manufacturing

The AxiChrom family is designed for straightforward scale-up, allowing methods developed at smaller volumes to be transferred to larger production columns with predictable performance. This makes the BPG 200/500 an excellent choice for organizations moving from process development to GMP manufacturing.

Optimized Flow Dynamics

The column’s internal geometry is engineered to maintain uniform axial compression and efficient flow paths, supporting consistent mass transfer and separation efficiency for complex mixtures in high-precision chromatography workflows.

Key Features

• Preparative and process-scale chromatography column

• Part of the Cytiva AxiChrom™ BPG column family

• Axial compression design for reproducible bed packing

• Compatible with ÄKTA™ systems and UNICORN™ software

• Designed for protein purification and biomolecule separation

• Supports pilot-scale to production-scale workflows

• Suitable for biotech and biopharmaceutical environments

Typical Applications

• Preparative HPLC and process chromatography

• Protein purification and fractionation

• Antibody and biologics downstream processing

• Biopharmaceutical manufacturing and scale-up

• Biotechnology and molecular biology research